omer uner
Classification: Unit 2
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Crash report
Biology WBI12/01 November 2021
1. Transcription regulation—RNA polymerase binding sites, promoter motifs, enhancer elements, epigenetic control via chromatin structure and DNA methylation
2. Post-transcriptional modification—splicing of introns, 5′ capping, polyadenylation signals, mRNA stability factors
3. Gene expression control—roles of transcription factors, miRNA, siRNA, mechanisms of repression and activation
4. Experimental techniques—PCR, RT-PCR, gel electrophoresis (SDS-PAGE, 2D), blotting methods (Northern, Western, Southern)
5. Protein interaction and analysis—co-immunoprecipitation, mass spectrometry, fluorescence microscopy, protein labeling
6. Genetic manipulation methods—CRISPR-Cas system, gene knockouts, reporter genes, mutagenesis, sequencing technologies
2. Post-transcriptional modification—splicing of introns, 5′ capping, polyadenylation signals, mRNA stability factors
3. Gene expression control—roles of transcription factors, miRNA, siRNA, mechanisms of repression and activation
4. Experimental techniques—PCR, RT-PCR, gel electrophoresis (SDS-PAGE, 2D), blotting methods (Northern, Western, Southern)
5. Protein interaction and analysis—co-immunoprecipitation, mass spectrometry, fluorescence microscopy, protein labeling
6. Genetic manipulation methods—CRISPR-Cas system, gene knockouts, reporter genes, mutagenesis, sequencing technologies