- Antibiotic resistance in bacteria, global health issue, research into antibacterial substances, honey as an alternative. - Manuka honey, antibacterial properties, methylglyoxal (MGO), hydrogen peroxide, Bacillus subtilis, aerobic respiration. - Measurement of bacterial growth, turbidity as an indicator, broth culture methodology, control experiments. - Preparation of dilutions, concentration calculations, stock solutions, 10% honey solution, graduated pipettes. - Independent and dependent variables, experimental design, prediction of results, statistical analysis using chi-squared test. - Gossypol toxicity in livestock, dietary impacts on milk production, genetically modified cotton plants, risk assessment of gossypol intake.